There are many in vitro methods for evaluating antioxidant activity. In this chapter, we describe an operationally simple cell-based assay, oxidative hemolysis inhibition assay (OxHLIA). OxHLIA is based on inhibition of free radical-induced membrane damage in erythrocytes by antioxidants. The advantage of this method is that it uses peroxyl radicals as pro-oxidants and erythrocytes as oxidizable targets so that the results obtained reflect biologically relevant radical-scavenging activity and microlocalization of antioxidants. We also present here a comparison of OxHLIA with other common methods (DPPH, ABTS •+, and ORAC assays). © 2010 Humana Press, a part of Springer Science + Business Media, LLC.
CITATION STYLE
Takebayashi, J., Chen, J., & Tai, A. (2010). A method for evaluation of antioxidant activity based on inhibition of free radical-induced erythrocyte hemolysis. Methods in Molecular Biology, 594, 287–296. https://doi.org/10.1007/978-1-60761-411-1_20
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