Freeze-fracture and thin-section electron microscopy and morphometry were used to characterize further the response of the rat medullary collecting duct to potassium depletion. In freeze-fracture replicas, principal cells and intercalated cells were identified based on the assumption that intercalated cells possess a high density of rod-shaped intramembrane particles in their luminal membranes. Potassium depletion caused an increase in the relative number of cells with a high density of rod-shaped particles from the control level of 22% to 31% after 2 wk and to 36% after 4 wk. The frequency of intercalated cells identified by thin-section criteria was, however, about 35% in controls and unchanged by potassium depletion. This suggests that intercalated cells can have 2 types of membrane morphology. In potassium depletion, all intercalated cells display a high density of rod-shaped particles in their luminal membranes. In addition, the luminal membrane area of intercalated cells increased more than 3-fold, and the density of their rod-shaped particles increased by 21%. These observations suggest that the intercalated cell and its rod-shaped particle may be involved with the potassium reabsorption that occurs in this nephron segment with potassium depletion.
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Stetson, D. L., Wade, J. B., & Giebisch, G. (1980). Morphologic alterations in the rat medullary collecting duct following potassium depletion. Kidney International, 17(1), 45–56. https://doi.org/10.1038/ki.1980.6