High constitutive level of NF-κB is crucial for viability of adenocarcinoma cells

Abstract

Most of cells exhibit low nuclear level of NF-κB. However, in some cell lines and tissues aberrantly activated NF-κB is playing an important role in cell motility, growth control and survival. Here we describe the result of decrease of constitutive NF-κB level in different adenocarcinoma cell lines. Treatment of mouse adenocarcinoma cell line CSML-100 with both synthetic (TPCK or PDTC) or natural (IκB-α) NF-κB inhibitors caused apoptotic death. Low doses of TPCK were harmless for CSML100 cells but sensitized them to TNF-induced apoptosis. Death of CSML100 cells in the presence of high concentration TPCK was not accompanied with significant changes in c-myc activity but strongly correlated with rapid decreasé in p53 level. Thus, mutual behavior p53 and NF-κB represented a unique feature of TPCK-induced apoptosis in CSML-100 adenocarcinoma cells.