Antioxidant activity of Ethanol Extract, n-Hexane fraction, Ethyl Acetate fraction and Water fraction of Garut Orange Leaves (Citrus reticulata Blanco)

Abstract

Antioxidants are compounds that are able to eliminate, cleanse, and resist radical effects. Antioxidants can stabilize free radicals by complementing the lack of electrons possessed by free radicals. Several studies have shown that citrus leaves of other species have been shown to have antioxidant activity. This study aims to determine the antioxidant activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of Garut orange leaves (Citrus reticulata Blanco) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. Garut orange leaves were extracted with ethanol 96% by macerator apparatus. Concentrated extracts were fractionated by liquid extraction method with n-hexane, ethyl acetate and water solvent. Furthermore, the ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of Garut orange leaves were tested for antioxidant activity using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. The antioxidant activity test results showed that ethanol extract of Garut orange leaves had better antioxidant activity with IC50 value of 42.925 ppm, followed by n-hexane fraction with IC50 value 46.156 ppm, ethyl acetate fraction with IC50 value 49.371 ppm and water fraction with IC50 55.662 ppm.