Optimization of extraction conditions and fiber selection for semivolatile analytes using solid-phase microextraction

Abstract

A group of 15 large volatile and semivolatile analytes (MW 92-499 amu) representing 13 organic classes are extracted with 9 different solid-phase microextraction fibers. The extraction efficiencies of the fibers for each of the analytes are compared. The influence of modifying the pH of the sample on the extraction efficiency of the fibers is shown. The effects of the size of the analytes with respect to fiber coating thickness and the relationship between fiber coating polarity and analyte polarity are discussed. In addition to fiber polarity and coating thickness, the different mechanisms by which the fibers extract are presented. A comparison of immersion and heated headspace extraction techniques for these analytes is shown.