Green fluorescent protein (GFP) is widely used as an excellent reporter molecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods.
CITATION STYLE
Yakhnin, A. V., Vinokurov, L. M., Surin, A. K., & Alakhov, Y. B. (1998). Green fluorescent protein purification by organic extraction. Protein Expression and Purification, 14(3), 382–386. https://doi.org/10.1006/prep.1998.0981
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