AtSig5 is an essential nucleus-encoded Arabidopsis σ-like factor

Abstract

Transcription of chloroplast genes is subject to control by nucleus-encoded proteins. The chloroplast-encoded RNA polymerase (PEP) is a eubacterial-type RNA polymerase that is presumed to assemble with nucleus-encoded σ-factors mediating promoter recognition. Recently, families of σ-factor genes have been identified in several plants including Arabidopsis. One of these genes, Arabidopsis SIG5, encodes a σ-factor, AtSig5, which is phylogenetically distinct from the other family members. To investigate the role of this plant σ-factor, two different insertional alleles of the SIG5 gene were identified and characterized. Heterozygous mutant plants showed no visible leaf phenotype, but exhibited siliques containing aborted embryos and unfertilized ovules. Our inability to recover plants homozygous for a SIG5 gene disruption indicates that SIG5 is an essential gene. SIG5 transcripts accumulate in flower tissues, consistent with a role for AtSig5 protein in reproduction. Therefore, SIG5 encodes an essential member of the Arabidopsis σ-factor family that plays a role in plant reproduction in addition to its previously proposed role in leaf chloroplast gene expression.