Background: Tomatoes provide a significant dietary source of the carotenoids, lycopene and ß-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties. Methods: We crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel 'orange-brown' fruit color was identified. Results: We confirm two SNPs, one in CYC-B and another in SGR gene sequence, associated with segregation of 'orange-brown' fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high ß-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.
CITATION STYLE
Manoharan, R. K., Jung, H. J., Hwang, I., Jeong, N., Kho, K. H., Chung, M. Y., & Nou, I. S. (2017). Molecular breeding of a novel orangebrown tomato fruit with enhanced beta-carotene and chlorophyll accumulation. Hereditas, 154(1). https://doi.org/10.1186/s41065-016-0023-z
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