The Sustainable Use of Delftia in Agriculture, Bioremediation, and Bioproducts Synthesis

Abstract

This book describes selected microbial genera from the perspective of their environmentally and commercially sustainable use. By focusing on their physiology and metabolism and combining historical information with the latest developments, it presents a multidisciplinary portrait of microbial sustainability. The chapters provide readers descriptions of each genus in the form of microbial models that move us closer to the goal of sustainability; selected chapters also include worldwide market information and lists of corresponding patents. Preface; Contents; About the Editor; Part I: Microbes in Sustainable Industrial Development; 1: Systems and Synthetic Biology Approaches for Metabolic Engineering of Pseudomonas putida; 1.1 Introduction; 1.2 Pseudomonas putida Is an Attractive SynBio chassis; 1.2.1 Central Metabolism in Pseudomonads as a Treasure Trove for Biotechnology; 1.2.1.1 Metabolism, Microbial Lifestyle, and Environment; 1.2.1.2 The Core Metabolism of Pseudomonas putida Is Characterized by a Cyclic Glycolysis; 1.2.1.3 Redox Metabolism; 1.2.2 Tools for Genetic and Metabolic Manipulation of Pseudomonads 1.2.2.1 Plasmids1.2.2.2 Transposon Vectors; 1.3 From Classical Approaches of Strain Manipulation Toward a Systems-Driven View of P. putida's Biology; 1.3.1 Genomics; 1.3.2 Transcriptomics; 1.3.3 Proteomics; 1.3.4 Genome-Wide Metabolic Reconstructions; 1.3.5 Metabolomics and Metabolic Flux Analysis; 1.4 Multi-omic, Systems-Based Biotechnology Approaches; 1.5 Conclusion; References; 2: Potentiality of Herbaspirillum seropedicae as a Platform for Bioplastic Production; 2.1 Introduction; 2.2 Genomic Organization of pha Genes in H. seropedicae SmR1 2.2.1 H. seropedicae SmR1 PhaC Proteins Belong to Different Phylogenetic Groups2.3 The Role of Phasins PhaP1 and PhaP2 on PHB Granule Formation in H. seropedicae; 2.4 Transcriptional Regulation of pha Genes in H. seropedicae; 2.5 Metabolic Engineering Strategies to Improve PHA Production in H. seropedicae; 2.5.1 Engineering NADPH Generation as a Strategy to Improve PHB Production in H. seropedicae; 2.6 Conclusions; References; 3: Engineering Hemicellulose-Derived Xylose Utilization in Saccharomyces cerevisiae for Biotechnological Applications; 3.1 Introduction 3.1.1 Saccharomyces cerevisiae3.2 Bioethanol Production; 3.2.1 Lignocellulosic Biomass; 3.2.2 Xylose Metabolism in Microorganisms; 3.2.3 Production of Ethanol by Engineering S. cerevisiae Utilizing Xylose as Sole Carbon Source; 3.3 Expression of Weimberg-Dahms Pathways and Production of Alternative Metabolites; 3.4 Transport of Xylose; 3.5 Strategies to Reduce the Effect of Fermentation Inhibitors in Lignocellulose Hydrolysates; 3.6 Conclusions; References; 4: Lactobacillus in the Dairy Industry: From Natural Diversity to Biopreservation Resources; 4.1 Introduction 4.2 Non-starter Lactic Acid Bacteria4.3 Role of NSLAB in Cheese Ripening; 4.3.1 Lactobacillus casei Group; 4.3.2 Lactobacillus plantarum Group; 4.3.3 Lactobacillus curvatus; 4.4 Lactobacillus as Biopreservation Resource; 4.4.1 Organic Acids; 4.4.2 Diacetyl and Acetaldehyde; 4.4.3 Hydrogen Peroxide (H2O2); 4.5 Bacteriocins; 4.5.1 Class I: Lantibiotics; 4.5.2 Class II: Non-lantibiotics; 4.5.3 Class III: Large Thermolabile Bacteriocins; 4.6 Most Important Lactobacillus spp. Bacteriocins; 4.6.1 Sakacin; 4.6.2 Plantaricins; 4.6.3 Helveticin