Abstract
Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.
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Powell, J. R., Blyton, M., Oberle, B., Powell, G. L., Rigg, J., Young, D., & Zanne, A. E. (2021). Extraction and purification of dna from wood at various stages of decay for metabarcoding of wood-associated fungi. In Methods in Molecular Biology (Vol. 2232, pp. 113–122). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1040-4_10
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