Interaction of immune cells and tumor cells in gold nanorod-gelatin composite porous scaffolds

Abstract

Composite porous scaffolds prepared by immobilization of photothermal nano-agents into porous scaffold have been used for both cancer therapy and tissue regeneration. However, it is not clear how the host immune cells and ablated tumor cells interact and stimulate each other in the composite scaffolds. In this research, a gold nanorod-incorporated gelatin composite scaffold with controlled spherical large pores and well interconnected small pores was fabricated by using ice particulates as a porogen. The composite porous scaffold was used for investigating the interaction between dendritic cells and photothermally ablated breast tumor cells. The composite scaffold demonstrated excellent photothermal property and the temperature change value could be adjusted by irradiation time and laser power density. The composite scaffold showed excellent photothermal ablation ability towards breast tumor cells. The photothermally ablated tumor cells induced activation of dendritic cells when immature dendritic cells were co-cultured in the composite scaffold. Consequently, the gold nanorod-incorporated gelatin composite porous scaffold should provide a useful platform for simultaneous photothermal-immune ablation of breast tumor.