CATECHIN CONTENT AND ANTIOXIDANT ACTIVITY OF SOME BREWED TEMPERATURE OF BLACK TEA SYRUP

Abstract

The catechin contents and the antioxidant activity of black tea syrup that brewed at some temperature still poorly understood. It has been investigated whether catechin contents and antioxidant activity of black tea syrup may be affected by hot or cold water. The temperature of 30°C, 60°C, and 90°C were used to brew the syrup, and then the catechin and the antioxidant were determined at each temperature by using DPPH radical scavenging activity method. Measurement of DPPH was conducted for 2 minutes reaction time. Catechin contents were determined by using HPLC method. The result of research showed that the catechin and the antioxidant activity affected by temperature tested. Five kinds of catechin derivatives detected in black tea syrup, EGC (epigallocatechin), C (catechin), EGCG (epigallocatechin gallate), EC (epicatechin) and ECG (epicatechin gallate). The highest DPPH scavenging activity of black tea syrup was showed by 30°C.