Abstract
Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described. The ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed. Correct folding of the synthesized protein is demonstrated and discussed in detail.
Cite
CITATION STYLE
Shirokov, V. A., Kommer, A., Kolb, V. A., & Spirin, A. S. (2007). Continuous-Exchange Protein-Synthesizing Systems. In In Vitro Transcription and Translation Protocols (pp. 19–55). Humana Press. https://doi.org/10.1007/978-1-59745-388-2_2
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
