Corrigendum: A Student’s Guide to Neural Circuit Tracing (Frontiers in Neuroscience, (2019), 13, (897), 10.3389/fnins.2019.00897)

Abstract

In the original article, there was an error: one section of our review considers reagents traditionally considered to be anterograde tracers (i.e., fluorescent or antigenic substances that are taken up by neuronal cell bodies at the site of application and transported to the synaptic terminals). The original text read: Emerging in the mid-1980s, dextran-based tracers, particularly biotinylated dextran amine (BDA), were rapidly adopted and remain one of the most widely used conventional anterograde tracers (Glover et al., 1986; Brandt and Apkarian, 1992; Veenman et al., 1992; Wouterlood et al., 2014). BDA enters injured neurons at the injection site, undergoes rapid anterograde transport and spreads evenly throughout the entire neuron, resulting in a Golgi-like level of staining detail (Köbbert et al., 2000; Lanciego andWouterlood, 2011;Wouterlood et al., 2014). Interestingly, while 10 kDa BDA travels mostly anterogradely, the 3 kDa form is a retrograde tracer (Reiner et al., 2000; Lanciego and Wouterlood, 2011). Like CTb, fluorophore-labeled dextran amine variants are now widely used instead of biotinylated versions that require histological processing for visualization, and a number of authors have used tetramethylrhodamine-conjugated dextran for juxtacellular labeling during electrophysiological recordings (Noseda et al., 2010; Dempsey et al., 2015).