Identification of the Aggregation Pheromone of the Brown-Winged Green Bug, Plautia stali SCOTT (Heteroptera: Pentatomidae)

Abstract

Identification of the aggregation pheromone responsible for the aggregation phenomena of the brown-winged green bug, Plautia stali SCOTT (Heteroptera: Pentatomidae) was attempted. Volatiles collected from adult males which attracted adults showed attraction activity under field conditions. Volatiles were then collected from a total of 180,000 adult males and the activity of the fractionated samples was field tested using water pan traps. A mixture of hexane and 5% ether in hexane fractions from Florisil LC showed attraction activity, and this was further purified with HPLC and GC. By GC-MS and NMR analysis (COSY), the chemical structure of the active compound was identified as methyl (E,E,Z)-2,4,6-decatrienoate. The attraction activity of the synthesized compound of this structure was confirmed by field tests.