HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro. © 2009 Humana Press.
CITATION STYLE
Rao, V. R., Eugenin, E. A., Berman, J. W., & Prasad, V. R. (2009). Methods to study monocyte migration induced by HIV-infected cells. Methods in Molecular Biology, 485, 295–309. https://doi.org/10.1007/978-1-59745-170-3_20
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