Pancreatic exocrine cells can be directly converted to insulin + beta cells by adenoviral-mediated expression of three transcription factors Pdx1, Mafa, and Ngn3 in the adult mouse pancreas (Zhou et al., Nature 455(7213):627–632, 2008). This direct reprogramming approach offers a strategy to replenish beta-cell mass and may be further developed as a potential future treatment for diabetes. Here, we provide a detailed protocol for inducing exocrine to beta-cell reprogramming in mice. We also describe key analyses we routinely use to assess the phenotype and function of reprogrammed cells.
CITATION STYLE
Cavelti-Weder, C., Li, W., Weir, G. C., & Zhou, Q. (2014). Direct lineage conversion of pancreatic exocrine to endocrine beta cells in vivo with defi ned factors. Methods in Molecular Biology, 1150, 247–262. https://doi.org/10.1007/978-1-4939-0512-6_17
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