Neuroproteomic technologies are crucial for a deeper understanding of molecular mechanisms underlying neuronal plasticity and pathologies of the central nervous system. For a comprehensive neuroproteomic analysis, high-resolution high-throughput techniques are indispensable. The most commonly used-system for 2D gel electrophoresis is based on the combination of isoelectric focussing and sodium dodecyl-sulphate-polyacrylamide gel electrophoresis. Nevertheless, the analysis of complex samples derived from biochemically prepared synaptic fractions raises some crucial challenges. Therefore, we describe the-general 2D gel electrophoresis procedure, including sample preparation, gel casting, electrophoresis-condition, and the detection of proteins on the gel with mass spectrometry compatible silver staining, sypro-ruby or colloidal Coomassie Brilliant Blue. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Smalla, K. H., & Wyneken, U. (2011). Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses. Neuromethods, 57, 95–113. https://doi.org/10.1007/978-1-61779-111-6_8
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