Monitoring I-SceI-induced double-strand break repair in DT40 cells

Abstract

The chicken B lymphocyte cell line DT40 is now a well-established system for studying DNA repair in vertebrates. Central to its utility is its ready genetic tractability allowing for the creation of isogenic mutants in any chosen gene. The combination of this genetic tractability with the I-SceI-induced double-strand break (DSB) repair assay, pioneered by the laboratory of Maria Jasin, has proven particularly powerful for dissecting the pathways of DSB repair in a vertebrate setting. © 2012 Springer Science+Business Media New York.