MRSA infection

Abstract

Molecular diagnosis of MRSA infection detecting mecA, spa gene and toxin gene by PCR including genotyping methods for MRSA nosocomial outbreak investigations are described. Restriction length polymorphism using pulsed-field gel electrophoresis(PFGE-RFLPs) with SmaI restriction enzyme is widely used for elucidating the infection route of MRSA. In Japan, MRSA is the major pathogen for nosocomial infections. These laboratory findings play an important role in infection control.