The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi, and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
CITATION STYLE
Kalendar, R., Boronnikova, S., & Seppänen, M. (2021). Isolation and Purification of DNA from Complicated Biological Samples. In Methods in Molecular Biology (Vol. 2222, pp. 57–67). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0997-2_3
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