Tackling formalin-fixed, paraffin-embedded tumor tissue with next-generation sequencing

Abstract

Most tumor samples available for clinical genotyping are formalin-fixed and paraffin-embedded (FFPE), but there has been relatively little published on the suitability of such samples for next-generation sequencing approaches. A new study by Wagle and colleagues shows that a combination of hybridization-capture and deep sequencing yields high-quality data from FFPE specimens. © 2012 American Association for Cancer Research.