Polymerase chain reaction

Abstract

The present review reports Polymerase chain reaction (PCR) microbiological techniques currently in use, and explore its applications to different aspects of medical field. PCR has rapidly become one of the most widely used technique in molecular biology which is rapid, inexpensive and simple means of producing relatively large number of copies of DNA molecules from minute quantities of source DNA material even when source DNA is of relatively poor quality. PCR involves preparation of the sample, the reaction mixture and primers, followed by detection and analysis of the reaction products.