Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain. Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ10 in isolated mitochondria. In the assay, mitochondrial suspensions are spiked with CoQ10-[2H6] internal standard, extracted with organic solvents, and CoQ10 quantifi ed by LC-MS/MS using multiple reaction monitoring (MRM).
CITATION STYLE
Itkonen, O., & Turpeinen, U. (2015). Mitochondrial coenzyme Q10 determination via isotope dilution liquid chromatography tandem mass spectrometry. Methods in Molecular Biology, 1264, 271–278. https://doi.org/10.1007/978-1-4939-2257-4_24
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