Neurotoxicity in Alzheimer's disease (AD) is associated to dishomeostasis of intracellular Ca2+ induced by amyloid β peptide (Aβ) species. Understanding of the effects of Aβ on intracellular Ca 2+ homeostasis requires preparation of the different Aβ assemblies including oligomers and fibrils and the testing of their effects on cytosolic and mitochondrial Ca2+ in neurons. Procedures for cerebellar granule cell culture, preparation of Aβ species as well as fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca 2+ in neurons are described. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Villalobos, C., Caballero, E., Sanz-Blasco, S., & Núñez, L. (2012). Study of neurotoxic intracellular calcium signalling triggered by Amyloids. Methods in Molecular Biology, 849, 289–302. https://doi.org/10.1007/978-1-61779-551-0_20
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