Coexpression of a plant NB-LRR-type resistance (R) gene and corresponding viral avirulent (Avr) gene introduced in Nicotiana benthamiana using Agrobacterium tumefaciens confers hypersensitive response (HR). Such Agrobacterium-mediated transient gene expression methods have contributed to the identification of new plant R genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus R genes from Solanaceous plants have been successfully identified and characterized molecularly.
CITATION STYLE
Tomita, R., Sekine, K. T., Tateda, C., & Kobayashi, K. (2019). Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes: Overview and Functional Analysis of Candidate Genes. In Methods in Molecular Biology (Vol. 2028, pp. 1–10). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9635-3_1
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