Effects of physicochemical factors on the adhesion to cellulose avicel of the ruminal bacteria Ruminosoccus flavefaciens and Fibrobacter succinogenes subsp. succinogenes

Abstract

Ruminococcus flavefaciens adhered instantly to cellulose, while fibrobacter succinogenes had the highest percentage of adherent cells after about 25 min of contact between bacteria and cellulose. Adhesion of R. flavefaciens was unaffected by high concentrtions of sugars (5%), temperature, pH, oxygen, metabolic inhibitors, and lack of Na+. In contrast, the attachment was affected by the removal of divalent cations (Mg2+ and Ca2+), the presence of celulose derivatives (methylcellulose and hydroxyethylcellulose), and cystine. Adhesion of F. succinogenes was sensitive to low and high temperatures, high concentrations of glucose and cellobiose (5%), hydroxyethylcellulose (0.1%), redox potential, pH, lack of monovalent cations, and the presence of an inhibitor of membrane ATPases or lasalocid and monensin. Cells of F. succinogenes heated at 100°C no longer were adherent. On the other hand, adhesion was insensitive to the lack of divalent cations (Mg2+ and Ca2+), the presence of 2,4-dinitrophenol, tetrachlorosalicylanilide, or inhibitors of the electron transfer chains. Adhesion of F. succinogenes seems to be related to the metabolic functions of the cell. External proteins and/or cellulases themselves might play a part in the attachment process. Several mechanisms are probably involved in the adhesion of R. flavefaciens, the main one being the interaction between the large glycocalyx and the divalent cations Ca2+ and Mg2+. Hydrophobic bonds and enzymes may also be involved.