Nickel pincer model of the active site of lactate racemase involves ligand participation in hydride transfer

Abstract

Lactate racemase is the first enzyme known to possess a metal pincer active site. The enzyme interconverts D-And L-lactic acid, which is important for the assembly of cell walls in many microorganisms. Here, we report a synthetic model of the active site of lactate racemase, which features a pyridinium-based SCS pincer ligand framework bound to nickel. The model complex mediates the dehydrogenation of alcohols, a reaction relevant to lactate racemization. Experimental and computational data indicate ligand participation in the dehydrogenation reaction.