Lawsonia inermis linnaeus leaf ethyl acetate extract evaluation on the kidneys of rats

Abstract

Leaf extract of Lawsonia inermis Linn. leaf extract (EAE) showed the ability to decrease the high of blood glucose level and to protect the hepar of diabetic rats at dose 1 g/kgbw. The aim of the present study was to evaluate the effect of EAE on normal rats organs, namely the kidneys. EAE was obtained by serial extraction using n-hexane and ethyl acetate (EAE). 10 rats were divided into two groups that treated with EAE 1.25 g/kgbw (Group I) and distilled water (DW) 10 ml/kgbw (Group II) as control group, orally, once daily for 14 days. After 14 days the rats were sacrified for histopathological evaluation of kidney using hematoxyilline-eosine staining. Grossly, no difference was found on the kidney of DW- or EAE-treated groups. However, microscopically, kidney of DW-treated group showed normal structure of glomerulus and tubulus renalis, whereas EAE-treated group showed the congestion of tubulus renalis. The present study concluded that EAE 1,25 g/kgbw caused the inflammation of the kidney.