Debunking the concept of dentinal tubule penetration of endodontic sealers: Sealer staining with rhodamine b fluorescent dye is an inadequate method

20Citations
Citations of this article
50Readers
Mendeley users who have this article in their library.

Abstract

The aim of this study was to investigate the suitability of rhodamine B dye staining of an epoxy resin sealer (AH Plus) and calcium-silicate-based sealers (Total Fill BC Sealer, BioRoot RCS) to represent the penetration depth of the sealers into dentinal tubules after root canal obturation. In a three-step process, (1) leaching of rhodamine B from sealers into a buffer solution, (2) passive penetration of leached rhodamine B into dentinal tubules, and (3) conformity of rhodamine B penetration assessed by confocal laser scanning microscopy (CLSM), and sealer penetration assessed by scanning electron microscopy (SEM), in root-canal-filled teeth, were evaluated. Rhodamine B dye massively leached out of Total Fill BC Sealer and BioRoot RCS into the phosphate-buffered saline (PBS). A pinkish coloration of AH Plus was found after contact with PBS. Leached rhodamine B dye passively penetrated dentinal tubules from all three sealers when placed on root dentin. No correlation was observed between sealer penetration in SEM and rhodamine B penetration in CLSM. Staining of sealers using rhodamine B is an inadequate method with which to evaluate sealer penetration depth into dentinal tubules, as it overestimates the penetration of sealers into root dentin tubules.

Cite

CITATION STYLE

APA

Donnermeyer, D., Schmidt, S., Rohrbach, A., Berlandi, J., Bürklein, S., & Schäfer, E. (2021). Debunking the concept of dentinal tubule penetration of endodontic sealers: Sealer staining with rhodamine b fluorescent dye is an inadequate method. Materials, 14(12). https://doi.org/10.3390/ma14123211

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free