Adipose tissue contains cell types other than adipocytes that may contribute to complications linked to obesity. For example, macrophages have been shown to infiltrate adipose tissue in response to a high-fat diet. Isolation of the stromal-vascular fraction of adipose tissue allows one to use flow cytometry to analyze cell surface markers on leukocytes. Here, we present a technical approach to identify subsets of leukocytes that differentially express cell surface markers. © 2008 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Brake, D. K., & Smith, C. W. (2008). Flow cytometry on the stromal-vascular fraction of white adipose tissue. Methods in Molecular Biology, 456, 221–229. https://doi.org/10.1007/978-1-59745-245-8_16
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