The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry.
CITATION STYLE
Cortella, L. R. X., Cestari, I. A., Soldera, M., Mazzetto, M., Lasagni, A. F., & Cestari, I. N. (2022). Surface Topography Obtained with High Throughput Technology for hiPSC-Derived Cardiomyocyte Conditioning. In IFMBE Proceedings (Vol. 83, pp. 119–126). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/978-3-030-70601-2_20
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