Isolation of cutaneous dendritic cells.

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Abstract

Cutaneous dendritic cells represent the first immunological interface with the environment and play a key role in the defense against pathogens that breach the skin. This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies.

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Helft, J., & Merad, M. (2010). Isolation of cutaneous dendritic cells. Methods in Molecular Biology (Clifton, N.J.), 595, 231–233. https://doi.org/10.1007/978-1-60761-421-0_15

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