Fluorophore-assisted carbohydrate electrophoresis (FACE) is a method in which a fluorophore is covalently attached to the reducing end of carbohydrates, thereby allowing visualization following high-resolution separation by electrophoresis. This method can be used for carbohydrate profiling and sequencing, as well as for the determination of the specificity of carbohydrate-active enzymes. Here, we describe and demonstrate the use of FACE to separate and visualize the glycans released following digestion of oligosaccharides by glycoside hydrolases (GHs) using two examples: (1) the digestion of chitobiose by the streptococcal β-hexosaminidase GH20C, and (2) the digestion of glycogen by the GH13 member SpuA.
CITATION STYLE
Robb, M., Hobbs, J. K., & Boraston, A. B. (2017). Separation and visualization of glycans by fluorophore-assisted carbohydrate electrophoresis. In Methods in Molecular Biology (Vol. 1588, pp. 215–221). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6899-2_17
Mendeley helps you to discover research relevant for your work.