During infection, the cytosolic detection of viral double-stranded RNA (dsRNA) leads to the oligomerization and activation of mitochondrial antiviral signaling protein (MAVS) and the subsequent production of type I interferon (IFN). Here, we describe a novel method of visualizing and quantifying the aggregation of MAVS in response to dsRNA stimulation or viral infection in vitro using confocal microscopy.
CITATION STYLE
Nguyen, T. A., Whitehead, L., & Pang, K. C. (2018). Detection and quantification of MAVS aggregation via confocal microscopy. In Methods in Molecular Biology (Vol. 1714, pp. 237–247). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7519-8_16
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