Analysis of splenic Gr-1int immature myeloid cells in tumor-bearing mice

19Citations
Citations of this article
14Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

It is known that the number of ImC, expressing myeloid markers, CD11 band Gr-1, increase with tumor growth and ImC play a role in the escape of tumor cells from immunosurveillance in tumor-bearing mice and cancer patients. However, the mechanisms by which ImC suppress immune responses in tumor-bearing mice have not been completely elucidated. In the present study, we investigated the function of splenic ImC freshly isolated from tumor-bearing mice and splenic ImC differentiated in vitro by GM-CSF. Freshly isolated splenic ImC were divided into two groups depending on Gr-1 expression, Gr-1 high (Gr-1 hi) and intermediate (Gr-1int). Freshly isolated splenic Gr-1int ImC, but not Gr-1hi ImC, from tumor-bearing mice reduced production of IFN-γ in CD8+ T cells, but neither splenic Gr-1int ImC nor Gr-1hi ImC isolated from naive mice did. Both Gr-1int and Gr-1hi ImC differentiated in vitro by GM-CSF inhibited production of IFN-γ in both CD8+ and CD4+ T cells. In addition, the differentiated Gr-1int ImC, one-third of which were CD11c+F4/80+ cells, and their culture supernatants suppressed proliferative responses of Tcells stimulated by CD3 ligation, but the differentiated Gr-1hi ImC and their culture supernatants did not. These results suggest that Gr-1int ImC are altered to immune-suppressive cells in tumor circumstances and that they are differentiated by GM-CSF progressively into CD11c+F4/80+ cells with further suppressive activity against T cells. © 2008 The Societies and Blackwell Publishing Asia Pty Ltd.

Author supplied keywords

Cite

CITATION STYLE

APA

Yamamoto, Y., Ishigaki, H., Ishida, H., Itoh, Y., Noda, Y., & Ogasawara, K. (2008). Analysis of splenic Gr-1int immature myeloid cells in tumor-bearing mice. Microbiology and Immunology, 52(1), 47–53. https://doi.org/10.1111/j.1348-0421.2008.00009.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free