We report a capillary electrophoresis method by field-amplified sample injection (FASI) for the separation and detection of free plasma arginine and dimethylated arginines. Protein elimination from plasma was performed by acetonitrile/ammonia precipitation. Supernatants were dried, reswollen in water, and directly injected on capillary without complex cleanup by solid-phase extraction and/or tedious sample derivatization procedures. Analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. Due to the stacking effects of electrokinetic injection, it is possible to perform a considerable on-line preconcentration of the analytes before running the electrophoresis. This procedure allows to reach a detection limit in real sample of 10 nmol/L for dimethylated arginines and 20 nmol/L for arginine. Recovery of plasma ADMA was 99-104 % and inter-day CV was less than 3 %. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Zinellu, A., Sotgia, S., Deiana, L., & Carru, C. (2013). Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection. Methods in Molecular Biology, 984, 131–138. https://doi.org/10.1007/978-1-62703-296-4_10
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