Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism. © 2012 Klose et al.
CITATION STYLE
Klose, C., Surma, M. A., Gerl, M. J., Meyenhofer, F., Shevchenko, A., & Simons, K. (2012). Flexibility of a eukaryotic lipidome - insights from yeast lipidomics. PLoS ONE, 7(4). https://doi.org/10.1371/journal.pone.0035063
Mendeley helps you to discover research relevant for your work.