Antibacterial Properties of Pit and Fissure Sealant Containing S-PRG filler on Streptococcus mutans

Abstract

The purpose of this study was to evaluate the antibacterial properties of a sealant containing S-PRG filler compared to those of two contemporary commercial sealants to determine the inhibition of bacterial growth in broth culture and biofilm formation using the CDC Biofilm Reactor. The BeautiSealant containing S-PRG filler, the fluoride releasing Clinpro TM sealant, which are known to have higher antibacterial effects, and the non-fluo-ride releasing Concise TM sealant were selected for this study. A Streptococcus mutans culture in BHI broth with-out sealant served as a negative control in the planktonic growth inhibition test. As a result, bacterial growth was inhibited in all three sealant groups compared to that in the control. The Clinpro TM sealant showed a signifi-cantly reduced number of CFUs compared to those of the BeautiSealant and Concise TM sealants. However, no significant difference was detected between the BeautiSealant and Concise TM sealants. The Clinpro TM sealant sig-nificantly decreased biofilm formation compared to that by the BeautiSealant and Concise TM sealants. No signifi-cant difference was observed between the BeautiSealant and Concise TM sealants. In conclusion, the sealant con-taining S-PRG filler had a less potent anti-bacterial property and increased biofilm formation capacity compared to those of the fluoride releasing Clinpro TM sealant. Abstract Ⅰ. Introduction Dental caries develop when bacterial plaque cannot be removed from the tooth surface. Approximately 90% of carious lesions are found in the pits and fissures of per-manent molar teeth