Plasmid specificity of the origin of transfer of sex factor F

Abstract

The ability of F like plasmids to promote transfer from the F origin of transfer was determined. Chromosome transfer was measured from plasmid derivatives of RecA- Hfr deletion strains which had lost all the F transfer genes but which in some cases retained, and in others had also lost, the origin sequence. ColV2 and ColVBtrp could initiate transfer from the F origin, but R100 1, R1 19, and R538 1 drd could not. These results can be correlated with the plasmid specificity of the tral components of the different plasmid transfer systems, supporting the hypothesis that the origin of transfer is the site of action of the tral product. Most F like plasmids, including R1 19 and R538 1 drd, could transfer ColE1, consistent with previous findings that the (plasmid specific) tral product is not necessary for ColE1 transfer by Flac; ColE1 transfer may be initiated by a ColE1 or host determined product. R100-1 and R136fin- could not transfer ColE1 efficiently, apparently because of differences residing in their pilus forming genes.