A compact time-gated instrument for QDs with low excitation energy and millisecond fluorescence lifetime as signal reporters, and its detection application

Abstract

Recently, bright quantum dots (QDs) possessing low energy for excitation and long fluorescence lifetime in milliseconds have been reported. These QDs such as Mn doped I(II)-III-VI nanocrystals are promising for highly sensitive time-gated sensing applications with a portable or small benchtop "personal" instrument because their unique optical properties not only ensure a high signal-to-background ratio in time-gated fluorescence-intensity (TGFI) measurement but also significantly simplify the TGFI measurement instrument design criteria. In this work, following up the research progress on these QDs, we developed a compact TGFI measurement instrument with high sensitivity and cost-effectiveness for these QDs (more specifically Mn:AZIS/ZnS QDs) as signal reporters. We applied the instrument for sensitive detection of copper(ii) ions in highly autofluorescent rum (alcoholic beverage) in a fluorescence quenching assay utilizing these QDs for signal transduction. The results from this work suggest that this instrument together with bright QDs with low-energy for excitation and long fluorescence lifetimes should have potential to not only convert many regular (non-time-gated) QD-based fluorescence assays to time-gated assays for higher sensitivities or lower LODs, but also facilitate the development of highly sensitive assays for in-field or point-of-care testing.