Evaluation of Bioactive and Antioxidant Activities Compounds Isolated from Heptapleurum Stellatum (Gaertn) Baill

Abstract

Recently, Heptapleurum stellatum is commonly known in India as Umbrella tree, has a wide range of medicinal uses. This study aimed to evaluate the phytoconstituents, antioxidant activities, total phenolic content and its compound characterization in leaves. Phytochemical analysis was indicated the presence of alkaloids, carbohydrates, steroids, saponins, glycosides, and terpenoids. The total phenolic content was measured by folinciocalteau assay. The total antioxidant capacity was estimated spectrometrically by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), ferric reducing antioxidant power (FRAP) and H2O2 assay. The methanol extracts exhibited maximum antioxidant activity. A high phenolic content of 0.357-2.004 GAE/mg and potent antioxidant activity (IC50 value=80 µg/mL) was observed in methanol leaf extract. High reducing ability in the FRAP assay (IC50 value=154.24 µg/mL) and H2O2 assay (IC50 value=110 µg/mL) was also observed in methanol leaf extract of H. stellatum. Bioactive compounds were screened using GC-MS analysis. The results suggest that H. stellatum has promising antioxidant activity and could serve as a potential source of natural antioxidants.