Study of antimicrobial resistance pattern of Escherichia coli and Klebsiella strains and multivariate analysis for water quality assessment of Tigris River, Baghdad, Iraq

Abstract

The present study aims to assess the pollutant impact from everyday untreated or partially treated industrial wastes, wastewater treatment plants and Baghdad Medical City wastewater discharge into Tigris River, Baghdad, Iraq. Water samples were collected from seven locations of the Tigris River near Baghdad Medical City in November 2018. Morphological characteristics and biochemical methods were used to characterize Escherichia coli and Klebsiella isolates revealing that the Tigris River accumulate different amounts of antibiotic-resistant E. coli and Klebsiella sp. isolates and that pattern of resistance is different in each site. E. coli was the predominant bacterial contaminant at site 1 which is near sewage of several hospitals in Baghdad Medical City. The influence of different water quality parameters (total dissolved solids, electric conductivity, alkalinity, turbidity, total hardness, calcium, magnesium, sodium, potassium, phosphate ion, nitrate, sulphate, chloride) and heavy metals (Cd, Zn, Co, Cu, and Ni) were investigated at bacterial contaminated site 1. Besides, physiological parameters (pH value and temperature) were applied. The results revealed that these parameters were within Iraqi standards levels with a slight increase in pH and temperature at site 1.