Extracellular signals are transmitted to intracellular targets thanks to a complex network of interacting proteins that regulate a large number of cellular processes. The signaling mechanisms that occur in CTLs are initiated by T cell receptors (TCRs) that recognize antigens presented in the groove of class I histocompatibility (MHC) molecules of target cells. The strength of this interaction and the consequent activation of intracellular mechanisms determine the fate of the developing T cells. The intracellular mediators of activation can be easily detected using a classical methodology such as western blot. Here we describe this technique bearing particular attention to these mediators using SNAP i.d., a newly developed protein detection system.
CITATION STYLE
Cox, S. N. (2014). Intracellular signaling of CTLs. In Methods in Molecular Biology (Vol. 1186, pp. 49–63). Humana Press Inc. https://doi.org/10.1007/978-1-4939-1158-5_4
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