Immuno-EM is a method that can determine the localization of a protein in a tissue at the ultrastructural level. Generally, membrane structures in immuno-EM specimens have very low contrast because fixation is performed without osmium. Here, by using high-angle annular dark field (HAADF)-scanning transmission electron microscopy (STEM) instead of transmission electron microscopy (TEM) for observation of immuno-EM samples, we demonstrate that photoreceptor disk membranes are clearly visible and that the procedures described in this article can be extended to visualize other membrane structures.
CITATION STYLE
Sakami, S., & Palczewski, K. (2019). Immuno-TEM/STEM in Retinal Research. In Methods in Molecular Biology (Vol. 1834, pp. 311–332). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8669-9_20
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