Haploid plants regenerated from the pollen callus of wheat (triticum aestivum L.)

Abstract

Anthers of Triticum aestivum L, cv. Chinese Spring and of some Japanese and Chinese wheat cultivars were cultured on the N6 basal medium supplemented with inositol, glutamine, serine and 2, 4-D after the treatment with low temperature (5°C) for 7 days. Haploid calluses were obtained from the pollen of Chinese Spring, Orofen, Dansheng 15 and (Orofen×Hsiaoyen)F1, and were maintained by continuous subcultures, in which calluses were transferred once for every 30 to 40 days on the PM-64 basal medium supplemented with 2mg/l of 2, 4-D. Many regenerated plantlets were obtained on the RM-64 basal medium supplemented with 1mg/l of IAA and kinetin. The regenerated plants were mostly haploid and green. A few plants from Orofen and Dansheng 15 were diploid. The haploid calluses remained capability of differentiation to plantlets for about a year, and normal plants were restored from them. These haploid calluses from wheat pollen may be useful in mutant selection studies. © 1981, The Genetics Society of Japan. All rights reserved.