We have recently developed a biochemical approach to isolate miRNA-bound mRNAs and have used this method to identify the genome-wide mRNAs regulated by the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.
CITATION STYLE
Subramanian, M., Li, X. L., Hara, T., & Lal, A. (2015). A biochemical approach to identify direct microRNA targets. Methods in Molecular Biology, 1206, 29–37. https://doi.org/10.1007/978-1-4939-1369-5_3
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