Elution of viruses by ionic and nonionic surfactants

Abstract

The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X- 100, respectively, eluted two viruses, φX174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when combinations of surfactants and binding membranes were matched (i.e., ionic-ionic or nonionic-nonionic).