Effect of Different Extenders and Storage Periods on Motility and Fertilization Rate of Rainbow Trout (Oncorhynchus Mykiss) Semen

Abstract

See, stats, and : http : / / www . researchgate . net / publication / 281449081 Effect Periods Rainbow (Oncorhynchus) Semen ARTICLE DOI : 10 . 13189 / ujar . 2013 . 010304 READS 7 3 , INCLUDING : Temel Recep 34 SEE Fikri Recep 16 SEE All - text , letting . Available : Temel Retrieved : 28 Abstract The effects of different extenders and storage periods on the motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen were evaluated after short - term storage . Semen was collected from anesthetized males by the abdominal massage . After determination of main semen characteristics , the pooled ejaculates were diluted with 4 different extenders at a ratio of 1 : 3 , and stored at 4ºC for 72 h . During preservation , spermatozoa motility (%) were determined every 12 h . Fertilization was carried out using the dry fertilization technique . The sperm - egg ratio was approximately 0 . 5x106 sperm / egg for optimum fertilization success . The highest motility (64 . 4±5 . 27%) and fertilization rate (94 . 3 ± 0 . 58 %) were obtained from semen stored with glucose based extender after 72 h storage . These results indicate that glucose based extender is a better preservative than the other solutions used in the study for the short term preservation of rainbow trout semen .