Temperature-dependent antibody kinetics as a tool in antibody lead selection

Abstract

Antibody-antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method. © 2012 Springer Science+Business Media, LLC.